FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44385559
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG566310.1275887952655953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32750.015.86691526
AGCTTAG186050.014.55027445
ATAGGGG201750.013.57233338
GGCATAG200350.013.47533435
GGGAGGT286000.013.2633783
GTCACTC286200.012.9096458
GGTCACT285250.012.7866387
ACTCTCC294900.012.58215811
GAGCTTA216450.012.49631944
TGTCGTT42700.012.48579628
TGGCATA220300.012.294909534
GAGGTCA309300.012.2059055
AGGTCAC310300.012.2045396
GGAGGTC311200.012.044674
TCACTCT321350.011.7706439
CATAGGG234750.011.6929337
GCTAAGT45950.011.60527514
CACTCTC326400.011.395410
GTCGTTC47000.011.15240429
ATATAGC60350.011.0365556
Produced by FastQC (version 0.11.5)