FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45098710
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161200.012.33940745
GGGAGGT242900.012.3195563
GGTCACT235600.012.1855717
ATAGGGG166350.012.07923938
GTCACTC243900.011.8354658
GGCATAG170450.011.74871735
GGAGGTC267000.011.27498154
ACTCTCC268400.010.88084611
GAGGTCA285700.010.5606215
TGGCATA195500.010.31174634
TCACTCT291500.010.2655829
GAGCTTA194300.010.26048744
CACTCTC285150.010.18646110
TAATCGG29400.09.8721864
CATAGGG208200.09.66201537
AGGTCAC319900.09.579296
GCATAGG213950.09.39182736
TGGGAGG242300.08.9050662
GCTCTGT338000.08.82696222
TAGGGGA327750.08.78095239
Produced by FastQC (version 0.11.5)