FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52782156
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG139200.012.541162538
GTTCTAT200100.012.1025141
GGCATAG143700.011.80974635
AGCTTAG150100.011.5718945
GTTGTCG24500.011.02572326
GGGAGGT234900.010.7690273
TCTATAG282850.010.7241463
CATAGGG163000.010.48767837
TGGCATA168750.010.15451534
GAGCTTA168750.010.14938144
CTATAGT300450.010.1390994
ATAGTGT306250.09.9714316
GCATAGG172750.09.92009136
GGAGGTC251000.09.906354
GGTCACT241450.09.818237
TTACTAG309100.09.81546214
GTCACTC242050.09.7753918
TATAGTG313050.09.7739585
ACTCTCC252450.09.63189611
TTCTATA329150.09.6118552
Produced by FastQC (version 0.11.5)