FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49042217
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1589560.3241207468251282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT278400.026.63159445
AGACCGC300200.024.939194
CAGACCG302400.024.8396323
GACCGCG320000.023.3678535
AGAACGA324350.022.87248644
ACCGCGT335850.022.3186426
CCGCGTT348150.021.535987
CGCGTTC349950.021.455968
AATACGG351700.021.17434132
ATACGGA351900.021.10480933
TACGGAG353150.020.98602534
TCAGACC365900.020.7740672
AAGAACG359100.020.62133443
CAATACG367600.020.31320831
CTCAGAC395250.020.2027111
GCGTTCT376500.019.9951049
ACGGAGA373700.019.86819535
CCCAATA384400.019.49631929
CGGAGAG385450.019.30956636
CCCCAAT413800.018.165528
Produced by FastQC (version 0.11.5)