FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49042217
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG598260.12198877550743678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG189900.015.49169945
ATAGGGG203400.014.5223538
GGCATAG201350.014.49117135
GGGAGGT334450.014.1566413
GGTCACT329350.013.7318037
GAGCTTA218150.013.57945344
GTCACTC341000.013.4033548
ACTCTCC352150.013.025310511
GGAGGTC360950.012.9509914
TGGCATA230400.012.80081434
AGGTCAC368300.012.7131556
CATAGGG240450.012.29420337
GAGGTCA384550.012.2102035
AGCATAG134350.011.86929245
TCACTCT395250.011.8379259
CACTCTC386800.011.82940710
GCATAGG258200.011.39675636
TGGGAGG288950.010.9518092
TAGGGGA413650.010.90431139
TCTCCCC432700.010.66855213
Produced by FastQC (version 0.11.5)