FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47395921
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT594800.12549603160997758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32500.025.9533966
CGGTATA47750.020.7736320
GTTCGAC40800.020.2887135
TCGACGA41700.020.0656457
AGACCGC171800.019.7705974
GACCGCG171750.019.7240055
ACCGCGT173800.019.5680836
CGACGAG41950.019.3026438
CAGACCG186550.018.436493
CGCGTTC186250.018.3325028
CCGCGTT185600.018.3239927
GAACGAT188750.018.09691245
TCAGACC224000.015.7362152
CGTTACG22350.015.699820521
GTCCGTT35750.015.6101661
CGTACGC38700.015.0541325
GCGTTCT232600.014.8244549
CCGTACG39150.014.7661524
GTTCCTA87300.014.7119115
AGTTCGA58500.014.5730114
Produced by FastQC (version 0.11.5)