FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49662873
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC155200.010.9587612
GTTGTCG26850.010.64014426
AGCTTAG144650.09.11982745
ACCCGGC65150.09.0482865
GGCATAG145400.09.02267335
ATAGGGG149100.09.01099738
AACTAAC140800.08.83872137
CAGGGAT214000.08.7459471
GGGAGGT222200.08.7278683
GGAGGTC213250.08.7040734
TCAGACG75700.08.2916262
GGGATCA216550.08.2179453
ACGGACA69400.08.167479518
TGGCATA164250.08.08274634
CAGACGT88550.07.92616841
GAGCTTA168150.07.912214344
ATATAGC52000.07.875546
CGTGGCG104000.07.8518241
TTAGGCC91850.07.719643640
CTTAGGC90900.07.69995139
Produced by FastQC (version 0.11.5)