FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45244824
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT668710.1477981216149719No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC526000.11625639211238836No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC524410.11590497069896881No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG509010.11250126644320685No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG504730.11155530188381327No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA479920.10607180171592666No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG464410.10264378528690929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA95000.020.15151415
AGACCGC171650.019.0732044
ACCGCGT182400.017.9572666
CAGACCG184300.017.7762643
CCTACTC110500.017.5698918
GACCGCG186400.017.5142465
GAACGAT188200.017.30342545
CGCGTTC193300.016.9213248
CCGCGTT193800.016.8776667
GCCGGCT183250.016.549671
TCACGCT192650.016.289478
TCAGACC213450.015.8230072
TTCACGC198400.015.7833557
CCGTACG43650.015.7224454
ACGCTCA208250.015.38249310
GCGTTCT214150.015.3578729
CGTACGC44250.015.3554865
AACATGC135950.015.2091935
CTCCGAT36050.015.1763291
CGCGTGT10700.015.1500631
Produced by FastQC (version 0.11.5)