FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55078554
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG185050.012.060657545
GGGAGGT252050.011.9119473
ATAGGGG192150.011.66182538
GGCATAG189400.011.60544535
GGAGGTC264050.011.3279784
GTTGTCG27700.010.47769626
TGGCATA217850.010.21376734
GAGCTTA214850.010.2097944
CATAGGG225750.09.67695437
GCATAGG233100.09.37182536
GTCAGGA196700.09.1527231
ACTCTCC332200.09.10921311
GGTCACT326750.08.8924447
GTCACTC338850.08.6943978
ATCGGTT50250.08.420396
TGGGAGG288250.08.2841562
ATAATCG45950.08.2288173
TAGGGGA348900.08.073297539
ATAACGC45250.07.955161639
AGGGGAG368550.07.954183640
Produced by FastQC (version 0.11.5)