FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49125663
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC69050.010.6004655
ATAGGGG102950.010.59874838
AGCTTAG100750.010.518784545
GGCATAG103900.010.19662335
CATAGGG114800.09.24906137
GGGAGGT203050.09.0622273
GCATAGG119700.09.03886536
TAGCGTA5556.366463E-118.91717337
GAGCTTA123550.08.66875244
CACCCGG88350.08.6418514
TCACCCG97800.08.61288453
TGGCATA125450.08.53470734
ATAACGC23550.08.50251939
GGAGGTC200600.08.3307984
CCGGACA91100.08.2484812
TAATCGG35700.08.2014154
ATAGCTC83200.08.13938639
GGTCACT188900.08.0492287
AGGGATC136150.08.039612
CGTAGGG55550.08.01823337
Produced by FastQC (version 0.11.5)