FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47681679
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1089410.22847559541684764No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1062180.22276480658325812No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG532620.11170328125400114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT290300.019.1166971
CCGGCTT342350.018.4356732
AGACCGC297350.018.1155974
GACCGCG297750.017.9388055
CAGACCG301200.017.9288643
ACCGCGT303200.017.7251746
TTCGACG31150.017.6211936
TTCACGC360500.017.3601197
TCACGCT374150.016.7147488
CCGCGTT321950.016.6857877
CGCGTTC326000.016.5198978
GTTCCTA103250.016.5150515
CGTTACG28800.016.40972121
CGGCTTC396950.016.3646763
TCAGACC346900.016.0080092
CGGTATA42700.015.44107820
ACGCTCA415750.015.18836310
CTCAGAC386550.015.1720761
TACGAGC46850.015.0291889
GTTCGAC32200.015.0226715
Produced by FastQC (version 0.11.5)