FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52939782
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG170250.014.27351345
GGCATAG184450.013.10139135
GGGAGGT294700.012.9689783
AGTTCAC200600.012.9522015
ATAGGGG190200.012.88268238
CTCAGTT216050.012.6402872
GGAGGTC291150.012.3390184
TTAGCCC179750.012.3145542
TCAGTTC227950.011.960673
GAGCTTA204100.011.91727544
TGGCATA207300.011.75528834
ACCCGGC75800.011.7521815
GGTCACT294450.011.7117747
GTTGTCG21300.011.6196826
GTCACTC310750.011.3650988
GTTCACC231750.011.1239086
CATAGGG225650.010.9285937
CCTTAGC170600.010.7588121
TTCTCAC226850.010.68082313
ATAGCTC79800.010.57369339
Produced by FastQC (version 0.11.5)