FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48748871
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1218980.2500529704575107No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1003080.20576476530092358No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG555320.11391443301322815No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT534490.1096415135439752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19650.023.106936
GCCGGCT312850.020.8718411
TCACGCT325600.020.7027958
GAACGAT258000.020.0254345
CCGGCTT392700.018.5312042
TTCACGC372950.018.2437787
AACGCTA362250.018.18885623
AGACCGC285350.018.1606434
ACGCTAC363900.018.12599824
CAGACCG295650.017.6803553
ACGCTCA391100.017.64361410
CGCTACC375100.017.56080225
CGGCTTC420350.017.5422383
AAAACGC380250.017.39746921
GAAAACG380900.017.33151820
TTCGGCG142300.016.996944
AAACGCT392650.016.86541722
CACGCTC407850.016.6875429
CGGTATA35750.016.59860220
AGAACGA319400.016.24992644
Produced by FastQC (version 0.11.5)