FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46864029
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG129000.010.72540438
AGCTTAG128650.010.5982345
GGCATAG133300.010.10811435
GGGAGGT219050.010.0997783
GTTGTCG20850.09.60366226
GAGCTTA145600.09.37988244
GTAACGA39300.09.278918
TAATCGG42450.09.2777154
GGTCACT223750.09.1932047
GTCACTC234650.09.1714238
GGAGGTC232650.09.0445964
ATCGGTT47100.08.8276676
TGGCATA157750.08.68405934
AGGGATC135500.08.6040542
ACTCTCC248650.08.49331511
ATAATCG48250.08.4893733
GGTAACG45750.08.46274817
CATAGGG161800.08.31487237
AATCGGT53450.08.2525665
GAGGTCA268350.08.1600325
Produced by FastQC (version 0.11.5)