FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43926228
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC76150.012.7641925
ATAGCTC73600.012.4423139
AGCTTAG92050.011.56157145
GGCATAG93500.011.09366935
TAGCTCT85250.011.0321840
TCACCCG99100.010.8071893
CATAGGG98950.010.61908237
ATAGGGG104500.010.35654138
TCAGACG82250.010.3129992
ACACGGA92150.010.30378116
CGGACAG96800.010.04125219
GCATAGG105550.09.9977136
CCGGACA107600.09.95346312
CACCCGG102900.09.8833124
GTCACTC159000.09.6508768
TGATAGC99600.09.623569537
GAGCTTA113000.09.517632544
GGTCACT158900.09.5011937
TTGATAG106800.09.1433336
TGGCATA114600.09.09046734
Produced by FastQC (version 0.11.5)