FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46316996
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA13900.013.11166145
GGCATAG121150.011.43900735
AGCTTAG125400.011.26807245
GTTGTCG21600.011.06144726
ATAGGGG131600.010.96304838
GGGAGGT197350.010.6639383
CACCGAA232800.010.29328845
TCACCCG65050.010.0820913
ACCCGGC61050.010.0426965
GAGCTTA143200.09.90515244
GGTCACT190200.09.8355777
GGAGGTC201550.09.7508034
CATAGGG147300.09.64655237
TGGCATA148500.09.44771734
GCATAGG150600.09.38869336
GCACCGA263150.09.19747644
TCTATAG279300.08.869923
AGGGATC120950.08.7092772
ACTCTCC217800.08.66242211
TTCTATA299850.08.614082
Produced by FastQC (version 0.11.5)