FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49788767
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG110250.012.9457245
ATAGGGG118950.012.12469838
GGCATAG120950.011.58932635
GAGCTTA134650.010.71625744
GGGAGGT269350.010.3417093
TGGCATA140050.010.0412934
ACCCGGC76000.09.8293845
ATAGCTC73400.09.53347639
CACCCGG124100.09.4827691
ACTCTCC279950.09.37743911
AGCATAG117500.09.31137745
GGAGGTC287650.09.2848164
GGTCACT269400.09.270917
GTCACTC277000.09.2686678
CATAGGG160950.09.04441637
GCATAGG168400.08.64446636
TAGCTCT83900.08.63527340
CGTGGCG135100.08.610741
TCAGACG90350.08.49179652
AGGGATC140600.08.4013182
Produced by FastQC (version 0.11.5)