FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49270007
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG153600.014.30932745
ATAGGGG168850.013.16356538
GGCATAG166550.013.14173435
GGGAGGT297350.012.0490123
GAGCTTA189650.011.75606344
TGGCATA188300.011.73128834
CATAGGG195600.011.54649737
GGAGGTC301850.011.1460074
GTTGTCG25200.011.06780426
GCATAGG208800.010.90273436
ACTCTCC309050.010.68285311
GGTCACT315300.010.3850137
ACCCGGC79100.010.2422175
GTCACTC325100.010.1548488
ATAGCTC75750.010.09799539
GCGTTCG24150.09.98239715
CACTCTC337900.09.81068310
TAGGGGA328850.09.80363639
TCACCCG93950.09.74931053
AGCATAG110200.09.26808745
Produced by FastQC (version 0.11.5)