FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54657175
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1802700.3298194610314199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT315200.027.57527245
AGAACGA368100.023.6374644
AGACCGC368550.023.570614
GACCGCG375900.023.0678335
ACCGCGT382700.022.7049836
CAGACCG391150.022.2835333
CCGCGTT391550.022.1684277
CGCGTTC399000.021.7602428
AAGAACG414500.020.95358743
TCAGACC434400.020.3446712
AATACGG428900.020.2338132
TACGGAG431350.020.12898634
ATACGGA432600.020.10745233
GCGTTCT445650.019.5533079
CTCAGAC480300.019.4482211
CAATACG450050.019.33327531
ACGGAGA463600.018.74826235
CGGAGAG472600.018.30150836
CCCAATA494300.017.68484329
CCCCAAT516250.017.03748928
Produced by FastQC (version 0.11.5)