FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54657175
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26650.014.69261426
ATAGGGG120200.014.34347838
AGCTTAG124450.013.72537245
GGCATAG130400.012.97750635
CATAGGG143200.012.02338337
GAGCTTA149400.011.46395344
TGGCATA155350.011.02362334
GCATAGG157650.010.99120636
GGGAGGT254150.010.8324383
TGTCGTT41750.010.40286528
ATATAGC46350.010.0931956
GGAGGTC270600.09.9662994
TCGCGCT47600.09.88135645
GCATTCG54350.09.8494593
AGGGATC152550.09.8193032
ACTCTCC267000.09.73928911
CGTAGGG84850.09.36334837
GTCACTC280200.09.360348
GCTAAGT45900.09.31209714
CGTAACG16000.09.28427842
Produced by FastQC (version 0.11.5)