FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51043441
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1872100.3667660258249439No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG921090.1804521760200297No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA843190.1651906657311759No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG813320.15933878752414046No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC544250.10662486488714584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG50200.024.3784246
GAACGAT402500.023.91709545
CGGTATA77150.020.73794720
GTTCGAC58050.020.6570845
CGACGAG56950.020.5802888
TCGACGA60750.019.8854527
CCGGCTT288900.018.683592
GCCGGCT246400.018.6818771
AGAACGA540500.017.83033644
TTCACGC283550.017.7715727
AACGCTA283450.017.75552223
ACGCTAC285000.017.65895524
TCACGCT287000.017.5265878
CGCTACC293800.016.99214725
AGACCGC565000.016.9285724
GACCGCG567900.016.765555
ACCGCGT570100.016.7508586
CAGACCG576900.016.6417773
CGTTACG26650.016.46726221
CGGCTTC333750.016.4559963
Produced by FastQC (version 0.11.5)