FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51043441
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA731390.14328775366065152No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG623100.12207249115513195No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG595680.11670059626270103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33300.013.79968526
GGGAGGT379650.012.818263
GGAGGTC377100.012.6478524
AGCTTAG271300.011.90476945
ATAGGGG282250.011.49116638
GAGGTCA415800.011.45939355
GGTCACT401450.011.4361247
GTCACTC412400.011.2631148
GGCATAG288150.011.162395535
AACTCTA117600.010.83248145
AGGTCAC444250.010.7947616
ACTCTCC435600.010.73255411
GAGCTTA303250.010.59686944
TGGCATA309500.010.40668934
TGTCGTT46700.010.32254928
TCACTCT455450.010.29680259
TGGGAGG338050.010.2446842
CACTCTC449000.010.23871910
CACCCGG121350.010.2320041
ATATAGC58900.09.623626
Produced by FastQC (version 0.11.5)