FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58888137
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG729810.1239315823490901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG50050.016.40889726
AGCTTAG267050.013.9391945
GCTAAGT63850.013.56483414
GGGAGGT418350.013.0509593
TGTCGTT64550.013.03674828
TATAGCT72850.012.9388877
ATATAGC76300.012.6781646
GGAGGTC427300.012.545954
ATAGGGG315400.011.87243438
GGTCACT452650.011.7141167
GTCACTC458300.011.5746118
GGCATAG321450.011.40390535
GAGCTTA327900.011.37978644
TCGTTCC74050.011.334558530
CGTTCCC77550.010.96822631
ACTCTCC485500.010.85199811
TGGCATA342750.010.80675734
GTCGTTC77950.010.79572929
TTGTCGT87250.010.75386427
CAATATA92900.010.6306844
Produced by FastQC (version 0.11.5)