FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44173972
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTCTGCCTGCTGTGACCTGCAGCTCCTCGTACTTGGTCTGGTACCA496120.11231048002656406No Hit
CTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCCA471610.10676196380981996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC99350.020.4726964
ACCGCGT100750.020.054476
GACCGCG100950.019.9253395
CAGACCG103750.019.7779483
CTCGTAT87400.018.8310071
CCGCGTT108500.018.5597977
CGTATTG69600.017.84453
CGCGTTC114200.017.51528
CTCGCGT14650.016.905291
GTCCGTT35100.016.8700451
TCGTATT84200.016.6475542
CCCGTAT29000.014.8286961
CGTTACG16700.014.68532421
CCCATGT294350.014.6401491
CTCATAT381900.014.6384351
CCCCCCT35550.014.1865241
GTCCGAT20050.014.0366421
CCCAGAT166800.014.0109931
TCAGACC150550.013.8987812
CTATCCC60800.013.8771817
Produced by FastQC (version 0.11.5)