FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44173972
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCCTCTCTCCTCCAGCCTCTCACACTCTCCTCAGCTCTCTCATCTCCT563930.1276611485152388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG47000.019.05309124
GTTGTCG25950.015.26003526
CTCTCGC100850.015.1938956
TCTCGCC99700.015.1653997
CCTCTCG97650.015.1388215
TCGCCTC99350.014.5847089
TCCCTCT323250.014.0260833
TTCCCTC240500.013.8560642
CTCGCCT114150.012.9894048
GGGAGGT199350.012.833473
CGCTCAC80600.012.56196729
ATAGGGG139750.012.5419638
TCGCTCA80750.012.45504228
ATATAGC39550.012.3455716
CACACTC333450.012.20641224
TCACACT340850.012.1460423
CTCACAC333600.012.13347822
GGCATAG146900.011.96214335
GGAGGTC221950.011.6990494
CGCTCGC86650.011.63293325
Produced by FastQC (version 0.11.5)