FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46217116
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT712530.1541701563550612No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG518510.11219003799371643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC171300.022.3140324
TTCGACG24000.022.028986
CAGACCG178950.021.5867463
GACCGCG176750.021.3713935
ACCGCGT185150.020.4988356
CCGCGTT196400.019.3246387
CGCGTTC197950.019.1841288
GAACGAT203700.018.56981345
TCGACGA29200.018.2601057
GCGTTCT216050.017.6394189
CGGTATA37450.017.60247820
TCAGACC226700.017.5064472
CCGGCTT207600.017.003822
CTCAGAC266050.016.829771
CGTTACG27250.016.76055721
GCCGGCT180950.016.6084581
GTTCGAC31000.016.4016785
TTCACGC207900.016.2429167
TCACGCT213600.015.8511348
CCGTACG38650.015.5418894
Produced by FastQC (version 0.11.5)