FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46217116
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27850.015.672626
GGGAGGT185000.012.8632143
ATAGGGG137700.012.66180438
GGCATAG134200.012.47233535
GGAGGTC193700.011.9659164
AGCTTAG148500.011.69567545
GAGCTTA155100.011.1400544
TGGCATA153400.011.13163234
TGTCGTT40050.011.12321928
ATATAGC45400.011.052686
GCTAAGT43250.010.81971614
GTCGTTC40850.010.6851329
CATAGGG160700.010.54132437
GTAACGA52000.010.51323618
GCATAGG163950.010.41472636
TAATCGG59600.010.2697764
TCGTTCC43500.010.13759830
ATCGGTT62350.09.7441936
CGTTCCC46400.09.64913431
TCAGACG65550.09.5833282
Produced by FastQC (version 0.11.5)