FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52394352
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG849840.16220068911244478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG257150.016.4782945
GGCATAG282900.015.03089335
ATAGGGG290900.014.67961438
GGTCACT412100.014.431037
GGGAGGT444550.014.29563
ACCCGGC98450.014.2630335
GAGCTTA295550.014.25294544
AGGTCAC426150.014.2192496
GAGGTCA433950.014.1153425
GTTGTCG30800.014.09825726
GTCACTC422050.014.0748198
TGGCATA304300.014.02559534
GGAGGTC436200.013.8469834
ACTCTCC449450.013.36665411
CATAGGG336800.012.69231237
CACTCTC474500.012.58520910
TCACTCT483500.012.49531659
TCACCCG120850.012.4390763
CGGACAC113850.012.17373813
GCATAGG355800.012.03974736
Produced by FastQC (version 0.11.5)