FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45469298
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG866070.19047358065655645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG22850.018.1160421
AGACCGC103850.016.8348124
ACGCGTC14250.016.7394340
GCCGGCT268150.016.6609671
CCGGCTT308400.016.2333982
CAGACCG109150.016.2235073
TCACGCT305200.015.9366188
GTCCGTT32450.015.5442481
TTCACGC315100.015.5287267
TTAGTTA46350.015.1921628
AAGCGCG16250.015.09034218
AACGCTA314500.015.00107423
GACCGCG117200.014.9363945
ACGCTAC318100.014.89517124
TTCGACG18400.014.7942856
TCGGCGC110350.014.4974345
ATTACGC19250.014.4915867
ACGCTCA346400.014.48278110
GTTCCTA76200.014.43709615
CGCTACC326850.014.42802625
Produced by FastQC (version 0.11.5)