FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45469298
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA576670.1268262377835699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA536580.11800929937383242No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG464460.1021480472383805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG105150.011.8319972
GTCACTC218250.010.93613158
AGCTTAG143800.010.92324145
GGTCACT219550.010.7996527
GGCATAG142500.010.73818435
GGGAGGT232700.010.7314243
ATAGGGG147550.010.6908438
GGAGGTC255200.09.91744
CGTGGCG180550.09.8938471
ACTCTCC248800.09.6937211
TCACTCT261950.09.4556969
TGGCATA166000.09.42133834
CAGACGT135200.09.3849763
GAGCTTA168100.09.30391844
AGGGATC158700.09.1154112
GTGGCGA198300.09.0082432
TAGGCTA70000.08.99891810
CACCGAA335600.08.92505845
CACTCTC270900.08.90258410
TATCCTA77900.08.8659645
Produced by FastQC (version 0.11.5)