FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48187681
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1530670.31764757469860394No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1076320.22335999111474156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT362250.022.8359361
AGACCGC253250.022.0595974
CAGACCG261600.021.475893
CCGGCTT422650.020.9803852
TCACGCT415450.020.9137028
TTCACGC430850.020.2028567
ACCGCGT278550.020.0220496
GACCGCG278550.019.9348185
CGGCTTC455900.019.809713
CCGCGTT285200.019.5238657
GAACGAT286550.019.36943645
ACGCTCA451400.019.33782410
CGCGTTC291700.019.104128
TTCGACG18700.019.0086236
TCAGACC304700.018.9188442
AACGCTA462050.018.42019723
ACGCTAC462000.018.40758124
CACGCTC492550.018.0876569
CGCTACC470850.018.0472825
AAAACGC482300.017.6561321
Produced by FastQC (version 0.11.5)