FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49555348
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG924120.18648239540160227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC100250.019.6613924
CAGACCG107500.018.7121473
CGTTACG22450.016.83710121
GACCGCG124100.016.027815
CCGTACG111550.015.7938034
CGTACGC114950.015.6202355
TTCGGCG116800.015.14098444
TACGCCA123650.014.57568557
TCCGTAC125100.014.3349293
ACCGCGT140500.014.2049916
GTACGCC127350.014.1699756
CCCGTAT30000.013.8820381
TCGGCGC128800.013.80020845
GACGGCA318400.013.48986838
CGGCAGT325600.013.3297740
ACGGCAG325050.013.27618439
TGACGGC331200.013.04324737
GTCCGAT23700.013.0129271
CTCGGTT78300.012.9663441
CGCCGCG137950.012.83593328
Produced by FastQC (version 0.11.5)