FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49555348
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG92750.012.5899282
GGCATAG87600.011.37814935
AGCTTAG90700.011.33651545
ATAGGGG88150.011.3326838
CATAGGG98650.010.21769537
CCGTACG19300.010.0256434
GAGCTTA104100.09.94208244
CAGACGT115450.09.8221563
GCATAGG102450.09.79478536
CGTGGCG159400.09.2951571
AGACGTG135400.08.84024054
TAGGCTA67950.08.74193310
ATCCTAG90800.08.7223256
CGTACGC24000.08.7185745
TATCCTA79650.08.7003895
TGGCATA115600.08.70007534
TGGCGAC172850.08.5780053
GTGGCGA170500.08.5642652
CGACCCG172100.08.5370496
GGCGACC177950.08.3827524
Produced by FastQC (version 0.11.5)