FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48851790
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2244130.4593751835910209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT320500.033.74551445
AGAACGA372050.029.09403844
AGACCGC397950.027.2317734
CAGACCG405300.026.8101083
AAGAACG421500.025.70213143
ATACGGA422100.025.54869333
AATACGG424700.025.4237632
GACCGCG429650.025.138795
TACGGAG430900.024.98515534
ACCGCGT434800.024.8979366
CCGCGTT436650.024.7747337
CGCGTTC438100.024.6927348
CAATACG443700.024.40606331
TCAGACC459900.023.9405562
GCGTTCT456450.023.7689849
CCCAATA461300.023.59695229
ACGGAGA456900.023.58799235
CGGAGAG467600.022.9856836
CTCAGAC507300.022.845671
CCCCAAT496250.022.1481828
Produced by FastQC (version 0.11.5)