FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50510614
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1033650.20464015741325178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT229800.021.57084845
AGACCGC255550.019.7500154
GACCGCG261600.019.2244475
CAGACCG263700.019.1909853
ACCGCGT262500.019.1549116
CGCGTTC276000.018.2093018
CCGCGTT276250.018.1933447
CGTTACG18200.017.6613221
AGAACGA282300.017.64482744
TCGGCGC115850.016.86233145
TCAGACC311250.016.5124682
ACGTTCG18000.016.1087066
GCGTTCT320200.015.7939859
TTCGGCG127500.015.602406544
AATACGG318650.015.58969832
ATACGGA320950.015.46396433
TACGGAG322250.015.42949534
AACGCTA161600.014.96813423
CCCGTAT35650.014.9134271
ACGCTAC164550.014.78263724
Produced by FastQC (version 0.11.5)