FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50510614
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG593490.11749807674086084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG193200.014.99284245
ACCCGGC104900.013.33939555
GGCATAG217950.013.25938335
TCACCCG112250.013.0070733
GAGCTTA229850.012.75325944
GGGAGGT357300.012.6808313
ATAGCTC106700.012.65616239
ATAGGGG238350.012.50224538
TGGCATA238200.012.19831134
TCAGACG112950.011.9505052
GGAGGTC356900.011.81887154
CCGTACG18600.011.49031454
CATAGGG257700.011.36260937
TAGCTCT119300.011.28173540
ACTCTCC376300.011.16770511
ACGGACA125800.011.051718
ACACGGA127000.010.84099116
GTCACTC382750.010.8325678
CGGACAG129600.010.6408619
GCATAGG281400.010.445621536
Produced by FastQC (version 0.11.5)