FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46214097
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT951810.20595663699758104No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG463960.10039360933526409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35800.026.836136
CGGTATA53650.022.52041220
GTTCGAC42950.022.1589645
AGACCGC209550.021.8820654
CAGACCG220150.020.9715463
TCGACGA47350.020.4801797
GACCGCG225050.020.3849875
CGACGAG46500.020.0319798
ACCGCGT229900.019.994226
GAACGAT232600.019.79099845
CCGCGTT235350.019.5217177
CGCGTTC234950.019.507078
TCAGACC270050.017.5546472
GCCGGCT164750.016.8942531
GCGTTCT274600.016.8378949
AGTTCGA58050.016.782534
CTCAGAC317150.016.4444681
CCGGCTT205900.016.3145562
TTCACGC201450.016.1836917
AGAACGA287150.016.03129644
Produced by FastQC (version 0.11.5)