FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48842928
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG546880.1119670794510927No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA539300.11041516593763584No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCC491670.10066349830624405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26300.013.5140826
GGGAGGT248650.013.3342073
AGCTTAG178600.013.18143545
GGAGGTC251500.013.0395994
ATAGGGG183900.012.87132438
GGCATAG187000.012.58476335
GAGCTTA205450.011.55737444
TGGCATA207300.011.46045334
TCAGACG80900.010.9901742
CGGGACG55150.010.72790517
GTCAGGA187850.010.6647981
TGTCGTT35100.010.38232928
CATAGGG230400.010.331470537
CGCTCTC73100.010.31149311
AGGGATC181150.010.3008292
GCGCTCT74000.010.27738610
AGCGCTC80550.010.1401539
TGGGAGG258950.09.9093462
GCATAGG243550.09.80136736
ATAACGC78850.09.76053639
Produced by FastQC (version 0.11.5)