FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53901049
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28250.014.256330526
AGCTTAG146700.013.61935245
GGCATAG147750.013.21801435
ATAGGGG152500.012.95383238
GAGCTTA168050.011.74178844
CATAGGG170550.011.49052737
GGGAGGT251150.011.4580463
TGGCATA174200.011.31435934
GGAGGTC254250.011.1059554
GCATAGG181050.010.73714436
TGTCGTT38200.010.54296828
ACTCTCC259400.010.4517811
ATATAGC49450.010.1463956
GAGGTCA289250.010.0343565
GGTCACT267300.010.0334547
GCGTCGT24450.09.938436511
GTCACTC274700.09.8942178
CACTCTC273800.09.82813110
GTCGTTC42400.09.65782129
TCACTCT291850.09.5903379
Produced by FastQC (version 0.11.5)