FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45133853
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT649370.1438764822493661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT148200.021.9808645
AGACCGC155500.021.5420594
TTCGACG19300.021.0981396
CAGACCG163600.020.626753
AGAACGA183800.017.79685444
ATACGGA191750.017.01592833
TACGGAG193400.016.82456834
AATACGG195200.016.78578232
GTCCGTT34000.016.7476771
GACCGCG205350.016.3454555
CAATACG205950.016.01893631
AAGAACG204800.015.96104943
CGTTACG14600.015.71973621
CGGTATA30050.015.42371320
ACCGCGT219200.015.3845216
GCGTTCT219800.015.3118199
CCGCGTT219250.015.3091867
CTCAGAC247350.015.2228861
CGCGTTC221100.015.2014418
TCAGACC229500.015.0273492
Produced by FastQC (version 0.11.5)