FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45133853
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG124550.014.56041345
GGCATAG138650.013.11214835
ATAGGGG142550.012.78498338
GGTCACT205750.011.8760597
GTCACTC205350.011.8444078
CATAGGG157350.011.63965337
GAGCTTA156700.011.60178344
ACTCTCC220700.011.17353511
GCATAGG165100.011.12052936
TAATCGG26050.011.0556714
TGGCATA171000.010.81578534
GGGAGGT242000.010.7665373
ATAGCTC60000.010.53749639
GTTGTCG20950.010.31025926
TCACTCT246000.010.2804849
CACTCTC246750.09.98479910
ACCCGGC71150.09.9297335
GAGGTCA263350.09.8253355
AGGTCAC262200.09.8083496
TCAGACG73450.09.3124662
Produced by FastQC (version 0.11.5)