FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57320430
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG596550.10407284104463278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG22850.018.60893221
CCCGGAT158650.016.9874021
GCCGGCT186500.016.9837681
TTCGACG15700.016.9049766
CGGATAC105900.016.5269953
AGACCGC90600.016.4376624
CCGGATA119800.016.0177842
AACGCTA202850.015.97814823
ACGCTAC204150.015.92065224
TCACGCT212350.015.8775778
GTCCGTT36800.015.7718311
CCGTACG92200.015.5180254
CGCTACC208850.015.44390425
TTCGCCA110800.015.28145514
CCGGCTT240500.015.1627562
CGTACGC95350.015.0997425
TTCACGC228800.014.7949217
AAAACGC221250.014.70384321
CAGACCG102900.014.6914313
CCCCCCT43850.014.67258551
Produced by FastQC (version 0.11.5)