FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57320430
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT251800.013.9097213
AGCTTAG202200.012.86311345
GGCATAG207500.012.28416435
ATAGGGG212300.012.17587138
ACTCTCC299700.011.30596411
GAGCTTA228900.011.29383344
GGAGGTC310350.011.154994
TCAGACG108700.011.1407452
GTTGTCG24950.011.0007626
GGTCACT310800.010.7115167
CATAGGG247000.010.46533437
TGGGAGG267400.010.4044372
CGTGGCG197500.010.3137281
TGGCATA251150.010.28356334
GTCACTC334650.010.0959868
GCATAGG256300.010.09437136
GTGGCGA205500.09.8252172
CACTCTC360950.09.31944610
TGGCGAC218900.09.2855273
CAGACGT130850.09.1682771
Produced by FastQC (version 0.11.5)