FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49960366
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG528450.10577384481130503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG190650.014.24278938
AGCTTAG191750.014.15706945
GGCATAG191300.013.91152635
GGGAGGT282400.013.8432273
GGAGGTC303100.012.7714414
GAGCTTA215200.012.61615344
TGGCATA222800.012.13671834
GGTCACT308350.012.0204927
GTCACTC306550.011.929598
CATAGGG238850.011.30255737
ACTCTCC341050.011.03931711
GCATAGG250200.010.83463636
GAGGTCA370750.010.6531635
GTTGTCG20350.010.61352526
TGGGAGG276750.010.5480772
CACTCTC356550.010.35084810
TCACTCT369100.010.3099179
TAGGGGA379200.09.75334839
CTGGGAG350800.09.7213331
GCTCTGT393300.09.54153122
Produced by FastQC (version 0.11.5)