FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40265623
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1617960.40182167304352895No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1241420.30830765986161446No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG952410.23653179288943327No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG689110.17114102518667101No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT635980.15794614676643648No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA540670.13427583127175258No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA535650.13302911021642458No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT533070.13238836513221217No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA527890.13110190794763066No Hit
TTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTG502570.12481366549326706No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA475640.11812557823829026No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG471850.11718432867659839No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT470260.11678945089214192No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC467760.11616857387255625No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC455360.11308902385541136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA109800.018.96362545
GGGAGGT378300.016.6223623
GAGGTCA406650.015.3700945
AGGTCAC409300.015.2551326
GGAGGTC413400.015.1351774
TATATAC62050.014.9401527
TGGGAGG328800.014.8817762
GGTCACT416500.014.8187097
GTCACTC423600.014.5172148
AACGCGC27950.014.48731115
CCATATA64250.014.392154
ATATATA66250.014.2985166
CTGGGAG367050.013.900281
TTCCATA83400.013.8921192
ACTCTCC456750.013.58672311
TCACTCT463550.013.5330539
CACTCTC469800.013.24764710
CATATAT72850.012.9713395
AGCTTAG363600.012.88958245
CATGCAT78100.012.8782279
Produced by FastQC (version 0.11.5)