FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51274032
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT726810.14175011631618906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC161850.021.7977284
GAACGAT166100.021.18530845
CAGACCG169350.020.9928873
TTCGACG17200.017.9208986
AGAACGA205800.017.31719444
ATACGGA208950.016.92691833
GACCGCG211250.016.7855685
CGTTACG19900.016.6217721
AATACGG213100.016.53392632
TACGGAG213600.016.49522234
ACCGCGT223200.015.9571176
AAGAACG221850.015.9426643
GCGTTCT225650.015.77389059
CAATACG226900.015.61757931
CGCGTTC230600.015.5231058
CCGCGTT230150.015.5045767
CTCAGAC271250.015.2226381
ACGGAGA242200.014.61242535
TCAGACC251550.014.5980942
CCCGTAT29950.014.5756681
Produced by FastQC (version 0.11.5)