FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51274032
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG692970.13515028426085157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG227900.015.08809945
GGGAGGT331950.014.9918663
GTTGTCG28550.014.26109826
GGTCACT341150.013.9436947
ATAGGGG245750.013.92337738
GGCATAG245900.013.813724535
GTCACTC346900.013.692438
GAGCTTA262100.013.03322144
AGGTCAC380550.012.9912626
GGAGGTC382550.012.9141124
ACTCTCC374400.012.84302711
TCACTCT382350.012.623089
GAGGTCA394050.012.5813075
TGGCATA279900.012.30438734
CACTCTC401050.011.92228610
TGGGAGG312000.011.7861492
CATAGGG303750.011.39812337
TGTCGTT38650.011.23254428
GCATAGG318800.010.90907236
ATATAGC46350.010.87505156
Produced by FastQC (version 0.11.5)