FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47083616
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG960120.20391806780515753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT207400.023.5604291
CCGGCTT248650.022.0529462
TTCACGC270950.019.8042817
CGTTACG18200.019.5319921
TCACGCT273000.019.4577778
CGGCTTC294850.018.9941793
ACGCTAC281050.018.48447624
AACGCTA281350.018.42474223
ACGCTCA296950.018.09301410
CGCTACC288300.017.99664725
GTTCCTA114400.017.9164615
CACGCTC301050.017.7643959
AAAACGC297400.017.58150121
GAAAACG303200.017.1857820
AAACGCT312750.016.76894622
GACCGCG95300.016.5034455
CCTACTC126950.016.3579518
GCACTAC88000.016.03104625
ACCGCGT100650.015.5580576
GTCCGTT35850.015.514581
Produced by FastQC (version 0.11.5)