FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47083616
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG156500.012.44034245
GGCATAG166600.011.60334235
ATAGGGG173000.011.46047438
GGGAGGT241900.011.3914623
GGAGGTC240650.011.0109864
CACCGAA275900.010.66238745
GCACCGA278400.010.55034644
GAGCTTA186800.010.362082544
TGGCATA187500.010.30951834
GTTCTAT162850.010.1289841
GTTGTCG20950.010.09473926
GGTCACT267500.09.88069257
GAGGTCA282800.09.7833475
CATAGGG198950.09.77336137
GTCACTC268000.09.761648
ACTCTCC279650.09.68554811
GCATAGG201750.09.64876436
CACTCTC274700.09.52415910
AGGTCAC301300.09.2796866
TCACTCT295000.09.2038429
Produced by FastQC (version 0.11.5)