FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49808547
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT679420.13640630793747105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG19650.019.35059521
TTCGGCG67950.017.81422444
TCGGCGC68800.017.65954245
GAACGAT187550.016.98713945
GCGTACG12450.016.80678423
GTCCGTT36000.015.6381661
CCCGTAT35600.014.1059771
CCGTACG88500.013.8819784
GCACTAC79500.013.867558525
CGTACGC89350.013.6995515
TTCGACG16500.013.4995216
GTTCCTA84050.013.46472215
CCCCCCT52250.013.0588421
CTCGGTT92900.013.041121
AGAACGA251250.012.7340844
ACCGCGT256800.012.7215116
GACCGCG257600.012.6830225
CGTACGA17200.012.55780824
AGACGCT262300.012.54077841
AGACCGC262350.012.4876964
Produced by FastQC (version 0.11.5)